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Veranstaltungskalender

Prof. Dr. Hans Hertz, KTH Royal Institute of Technology, Stockholm
Laboratory water-window x-ray microscopy
Veranstaltungsart: Physikalisches Kolloquium
Zeit: 19.11.2018, 16:15 - 17:30 Uhr
Ort: Hörsaal 1, Abbeanum
Veranstalter: Physikalisch-Astronomische Fakultät
Ansprechpartner:

Dekanat

Max-Wien-Platz 1

07743 Jena

Tel. 03641 9-47001

Details:


X-ray microscopy allows high-spatial-resolution 3D imaging of intact cells with natural contrast. We developed the first high-resolution laboratory x-ray microscope operating in the water-window (E=284-540 eV).1 The present version of the microscope relies on a liquid-nitrogen-jet-target laser-plasma x-ray source, multilayer condenser optics, zone-plate imaging optics and cryogenic sample handling.2 This microscope design has enabled proof-of-principle biological imaging in 2D and 3D with synchrotron-like image quality.3

Recent improvements have focused on shorter exposure times and higher reliability to allow routine tomographic cryo imaging in cell biology. Improved source power4 and higher multilayer mirror reflectivity5 allows 2D projections to be recorded in 10-20 seconds. Improved stability of source6 and system allows repeated tomographic imaging with up to hundred projections during typically 1 hour total data acquisition time. We demonstrate the microscope performance by imaging autophagy-relevant behavior of starving HEK cells and interaction between NK-cells and HEK target cells,5 as well as assessment of virus infection dynamics in amoebas7. Improved data acquisition strategies and tomographic routines show promise for further improved image quality.8

1Berglund et al, J. Microscopy 197, 268 (2000)
2Takman et al, J. Microsc. 226, 175 (2007); Bertilson et al, Opt. Lett. 36, 2728 (2011).
3See e.g., Hertz et al, J. Struct. Biol. 177, 267 (2012).
4Martz et al Opt. Lett. 37, 4425 (2012
5Fogelqvist et al, Sci. Rep. 7, 13433 (2017).
6Fogelqvist et al, J. Appl. Phys. 118, 174902 (2015).
7Kördel et al, ms in preparation
8Selin et al Opt. Lett. 40, 2201 (2015).

 

 

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